Hauv preclinical bioanalysis, kev xaiv ntawm biological matrix- ntshav los yog ntshav plasma- yog ntau tshaj qhov kev txiav txim siab niaj hnub. Nws cuam tshuam ncaj qha rau cov ntaub ntawv raug, kev soj ntsuam kev ntseeg tau, thiab kev txhais lus ntawmPharmacokinetic (PK)thiabpharmacodynamic (PD)tshwm sim. Ntawm Prisys Biotech, CRO preclinical tshwj xeeb hauv cov qauv tsis yog -tib neeg primate (NHP) thiab kev tshawb fawb tsiaj loj, peb hais txog kev xav txog kev xaiv matrix raws li cov kauj ruam hauv paus hauv kev txhim kho tshuaj thaum ntxov. Kab lus no qhia txog qhov sib txawv ntawm lub cev, qhov cuam tshuam rau kev txheeb xyuas, thiab kev xav txog kev xav tom qab kev xaiv matrix.
Nkag siab txog Biological Matrices
Ob leeg ntshav thiab ntshav yog muab los ntawm tag nrho cov ntshav, tab sis lawv cov biochemical muaj pes tsawg leeg sib txawv heev vim muaj los yog tsis muaj cov yam tseem ceeb.
Serumyog cov kua uas tau tom qab cov ntshav tau txhaws. Thaum lub sij hawm coagulation, fibrinogen hloov mus rau fibrin, tsim ib lub mesh uas cuab cov ntshav. Tom qab centrifugation, cov kua dej uas tseem tshuav -serum- muaj cov proteins, cov tshuaj hormones, nucleic acids, thiab lwm yam analytes tab sistsis muaj fibrinogen thiab qee yam ua kom ntshav txhaws.
Plasma, ntawm qhov tod tes, yog tau los ntawm kev ntxiv cov tshuaj tiv thaiv coagulants (piv txwv li, EDTA, heparin, citrate) los tiv thaiv cov ntshav txhaws, ua raws li centrifugation. Plasma khaws ciafibrinogen thiab coagulation yam, muab kev sawv cev ze dua ntawm covnyob rau hauv vivo physiological xeev.
Matrix Xaiv: Vim Li Cas Nws Tseem Ceeb
Kev xaiv ntawm cov ntshav thiab plasma tsis yog kev txiav txim siab. Feem ntau cov analytes muaj ntau qhov sib txawv nyob ntawm qhov matrix. Kev sib xyaw matrices hauv tib txoj kev tshawb fawb tuaj yeem cuam tshuam qhov kev ncaj ncees, uamatrix standardizationqhov tseem ceeb rau kev sib piv siv tau thiab kev ua raws cai.
Cov ntsiab lus tseem ceeb:Rau ib qho kev tshuaj ntsuam xyuas, ib txwm siv lub matrix zoo ib yam thoob plaws qhov kev kawm.
Qhov zoo ntawm Plasma
Plasma tau txais txiaj ntsig ntau ntxiv hauv bioanalysis niaj hnub no, tshwj xeeb tshaj yog rau cov kev tshawb fawb loj -tsiaj thiab NHP, vim yog cov haujlwm hauv qab no thiab kev tshawb fawb zoo:
1. Kev ua qauv nrawm dua
Plasma tuaj yeem centrifuged tam sim ntawd tom qab sau. Nyob rau hauv sib piv, serum xav tau ib30-feeb clotting lub sij hawm, uas tuaj yeem ntev dua rau cov neeg mob uas muaj coagulopathies lossis nyob rau hauv kev kho mob anticoagulant. Kev ua tsis tiav ntawm cov ntshav tuaj yeem ua rau microclots cuam tshuam nrog kev ntsuas qis lossis ntsuas ntsuas.
2. Yield siab dua
Plasma feem ntau suav txog ~ 55% ntawm tag nrho cov ntshav ntim, thaum cov ntshav tawm los yog ~ 15-20% qis dua. Hauv cov kev tshawb fawb uas cov qauv ntim tsawg tsawg-xws li hauv cov qauv menyuam yaus lossis hemophilia-plasma tso cai rauntau dua analytical yooj.
3. Txo kev cuam tshuam los ntawm Clotting
Cov txheej txheem ua kom ntshav hloov pauv cov qib tshuaj ntsuam xyuas los ntawm kev ua kom cov metabolism hauv cellular thiab tso cov ntsiab lus intracellular. Piv txwv li,potassium, LDH, thiab ASTCov concentrations feem ntau yog nce siab hauv cov ntshav. Conversely, analytes nyiamqabziblos yogplateletsTej zaum yuav txo qis thaum lub sij hawm txhaws, ua rau ntshav plasma xaiv ruaj khov rau qee lub hom phiaj.
4. Kev pheej hmoo tsawg dua ntawm Kev Tshawb Fawb Kev cuam tshuam
Cov qauv kuaj ntshav feem ntau muaj fibrin strands lossis microclots uas tuaj yeem thaiv cov cuab yeej tshuaj ntsuam xyuas lossis cuam tshuam nrog immunoassays. Plasma zam qhov teeb meem no tag nrho thaum ua tiav.
Qhov zoo ntawm Serum
Txawm hais tias cov txiaj ntsig ntawm plasma, cov ntshav tseem yog tus qauv matrix hauv ntau cov txheej txheem tsim thiab muaj nws tus kheej zoo:
1. Tsawg Risk ntawm Cellular Contamination
Cov qauv ntshav tau dhau los ua cov txheej txheem txhaws tag nrho, tshem tawm cov ntsiab lus ntawm tes. Piv txwv li,plasma tuaj yeem khaws cov platelets lossis cellular fragmentsyog tias centrifugation yog qhov zoo tshaj plaws, uas tuaj yeem ua rau cov qauv kev ncaj ncees nyob rau lub sijhawm lossis nrog khov -thaw cycles.
2. Stability ntawm Specific Analytes
Tej analytes qhia tau hais tiaschemical stability ntau duahauv cov ntshav ntau dua li ntshav ntshav. Piv txwv li, cov piam thaj yuav ruaj khov dua hauv cov ntshav nyob rau hauv ib puag ncig, txawm hais tias qhov no yog kev tshuaj ntsuam xyuas- tshwj xeeb.
3. Tsis cuam tshuam los ntawm Anticoagulants
Kev npaj plasma yuav tsum muaj cov tshuaj tiv thaiv coagulants, qee qhov (piv txwv li, EDTA, heparin) tuaj yeemcuam tshuam nrog cov kev tshawb nrhiav hauv qab, tshwj xeeb yog immunoassays. Serum zam qhov kev pheej hmoo no tag nrho.
4. Yooj yim ntawm Hla-Study Comparison
Vim nws cov keeb kwm nthuav dav, ntau qhov kev siv ntau yam, cov txheej txheem txheej txheem, thiab cov ntaub ntawv qub txeeg qub teg yog raws li cov ntshav qab zib. Rau qee qhov kev pab cuam, qhov no yooj yimbenchmarking thiab regulatory xa.
Xaiv Txoj Cai Matrix: Kev xav txog tswv yim
Yog tsis muaj universal teb. Matrix xaiv yuav tsum ua raws li:
- Target Analytes Properties
- Cov txheej txheem analytical xav tau
- Tshawb xyuas lub sijhawm teem sijhawm thiab cov qauv tswj kev txwv
- Kev txwv ntim (tshwj xeeb tshaj yog hauv NHPs lossis kab mob qauv)
- Kev tswj hwm lossis keeb kwm ua ntej
Cov lus pom zoo: Tsis txhob sib tov matrices hauv tib qhov project. Thaum xaiv, siv tib lub matrix tsis tu ncua kom tsis txhob muaj qhov sib txawv thiab ua kom cov ntaub ntawv sib piv.
Kev xaiv Matrix yog qhov tseem ceeb tab sis feem ntau tsis pom kev txiav txim siab hauv preclinical bioanalysis. Ntawm Prisys Biotech, peb pab cov neeg tsim tshuaj yeeb ua pov thawj-raws li kev xaiv uas ua kom muaj kev txheeb xyuas qhov sib xws thiab kev txhais lus ua tiav. Txawm hais tias siv plasma los xav txog hauv vivo physiology lossis ntshav qab zib kom haum nrog cov ntaub ntawv keeb kwm yav dhau los, peb kho peb cov tswv yim bioanalytical rau cov kev xav tau tshwj xeeb ntawm txhua txoj kev tshawb fawb.
Hais txog Prisys Biotech
Prisys Biotech yog CRO preclinical tshwj xeeb hauv kev loj -tsiaj tshuaj thiab kev tshawb fawb kev txhais lus, nrog rau kev tsom mus rautsis yog-neeg primates (cynomolgus liab). Peb cov kev pabcuam bioanalytical sib xyaw ua ke yog tsim los txhawb siab -PK, PD, thiab kev tshawb fawb txog tshuaj toxicology rau IND-pab thiab ntxov- theem kev txhim kho.
