NHP Intrathecal (IT) Txhaj Tshuaj & CSF Sampling Services|Prisys Biotech
Technical Overview: Intrathecal (IT) Administration thiab CSF Sampling hauv NHPs
Hauv txoj kev loj hlob ntawm CNS-cov tshuaj kho cov phiaj xwm- xws li Antisense Oligonucleotides (ASOs), gene therapies, thiab biologics-surpassing the blood-brain barrier (BBB) tseem yog ib qho nyuaj heev.Prisys Biotechmuab tus qauv, siab -precision Intrathecal (IT) Kev Tswj Xyuas thiab Cerebrospinal Fluid (CSF) Cov kev pabcuam hauvTsis yog -Human Primates (NHPs)los txhawb pharmacokinetic (PK) thiab pharmacodynamic (PD) profiling.
1. Dual-Route Access: Cisterna Magna thiab Lumbar Puncture
Peb lub platform siv ob txoj hauv kev anatomical tseem ceeb los xyuas kom meej qhov kev xa tshuaj hauv zos thiab kev saib xyuas lub cev:
- Ultrasound-Kev taw qhia Cisterna Magna (CM) Puncture:Peb ntiav -lub sij hawm ultrasonography tiag tiag kom pom qhov chaw subarachnoid. Qhov no ua kom cov koob nkag mus rau dura mater precisely, txo qhov kev pheej hmoo ntawm kev raug mob mus rau lub hlwb lossis txha caj qaum.
- Lumbar Puncture (LP):Los ntawm optimizing spinal flexion, peb cov kws kho mob ua kom qhov chaw intervertebral kom txhim khu kev qha lumbar nkag. Txoj kev no feem ntau yog siv rau bolus dosing lossis catheter kev koom ua ke.
2. Advanced Catheterization thiab Chronic Porting
Rau cov kev tshawb fawb ntev uas xav tau kev kuaj mob ntau zaus lossis kev txhaj tshuaj tas mus li, Prisys muaj kev cuam tshuam tshwj xeeb:
- Subcutaneous IT Porting:Implanted ports tso cai rau rov nkag tau nrog cov tsiaj tsawg tsawg. Cov chaw nres nkoj tau khaws cia nrog heparinized saline kom ntseeg tau ntev - lub sijhawm patency.
- Indwelling Catheters:Precision tso catheters (nrog beveled xaus qhia caudally) enables nruam CSF sau.
- CSF Volume Management:Txhawm rau tswj lub cev intracranial siab, peb nruj me ntsis tswj cov ntim ntim (feem ntau<2ml/day) and utilize artificial CSF (aCSF) supplementation where necessary.
3. Kev Tswj Xyuas Zoo thiab Kev Tshawb Fawb Kev Tshawb Fawb
Kev ncaj ncees ntawm CNS cov ntaub ntawv nyob ntawm qhov purity ntawm cov biofluids sau. Prisys ua raws li cov qauv hauv cov qauv nruj nruj:
- Kev Tiv Thaiv Kab Mob:Txhua tus qauv raug tshuaj xyuas kom muaj ntshav paug. Hemolyzed cov qauv raug sau tseg thiab cim los tiv thaiv kev cuam tshuam nrog LC-MS/MS lossis ELISA- raws li kev soj ntsuam.
- Standardized ua:Peb muab kev txhawb nqa kev sib koom ua ke, suav nrog siab -rhiab LC-MS/MS tsom xam rau cov ntawv xeem (TA) kom tsim tau qhov tseeb CSF concentration- lub sijhawm nkhaus.
- Kev txheeb xyuas qhov ua tiav:Qhov kev tso kawm raug raug raug txheeb xyuas tas li los ntawm qhov muaj qhov pom tseeb, qhov tshwm sim ntawm CSF ntws ua ntej kev noj tshuaj lossis kev kuaj mob.
4. Cov ntawv thov hauv NHP Kev Tshawb Fawb
- Pharmacokinetics:Kev txiav txim siab ntawm cov tshuaj ib nrab -lub neej thiab kev faib tawm hauv CNS compartment.
- Biomarker Discovery:Longitudinal saib xyuas ntawm neuroinflammatory lossis neurodegenerative markers hauv CSF.
- Kev Nyab Xeeb Pharmacology:Kev soj ntsuam ntawm cov ntaub so ntswg hauv zos tolerability thiab neurological cuam tshuam ntawm IT- tswj cov tebchaw.
Technical Specification Summary
| Feature | Specification / Txoj kev |
| Hom | Cynomolgus Macaque (Macaca fascicularis), Rhesus Macaque |
| Kev taw qhia | Real{0}}time Ultrasound (Cisterna Magna) |
| Dosing Volume | Tag nrho ~ 18ml / tsiaj muaj peev xwm; tswj cov kev txwv txhua hnub |
| Sampling zaus | Txog li 7+ cov qauv hauv 8-24 teev (nyob ntawm kev kawm) |
| Kev them nyiaj yug Analytical | Integrated LC-MS/MS, Protein Analysis |
Cim npe nrov: NHP Intrathecal (IT) Txhaj Tshuaj & CSF Sampling Services|Prisys Biotech, kev tshawb fawb, kev tshawb fawb, liab, tshuaj kho noob
Koj Tseem Yuav Zoo Li
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