siRNA (me me cuam tshuam RNA) tau txais txiaj ntsig tseem ceeb hauv kev kawm thiab kev lag luam kws tshuaj vim nws lub peev xwm hauv kev hloov pauv kev kho mob ntawm ntau yam kab mob. Hauv kev tshaj tawm tsis ntev los no los ntawm Beijing Institute of Technology thiab Suzhou Xuanjing Biotechnology Co., cov kev cov nyom thiab cov tswv yim hauv siRNA tshuaj txhim kho tau raug tshuaj xyuas, muab kev nkag siab zoo rau cov kws tshawb fawb thiab cov kws kho mob. Qhov blog no tshawb txog peb qhov kev sib tw tseem ceeb - Nkag mus, Kev khiav tawm, thiab Kev Ua Haujlwm - hauv siRNA kev txhim kho tshuaj thiab kev koom tes ntawm Prisys Biotech, tus neeg ua yeeb yam tseem ceeb hauv kev tshawb fawb tsis yog - tib neeg primate (NHP) kev tshawb fawb thiab Daim Ntawv Cog Lus Tshawb Fawb (CRO) cov kev pabcuam.
Qhov Kev Sib Tw Nkag Nkag:
Kev ua kom zoo ntawm siRNA hauv lub hom phiaj ntawm lub cev / cov nqaij mos thiab tom qab ua haujlwm hauv lub hlwb ua rau muaj teeb meem loj rau cov tshuaj siRNA. Prisys Biotech lees paub cov kev cov nyom no thiab koom nrog hauv kev tsim cov tshuab xa tawm tshiab los txhim kho siRNA lub hom phiaj thiab kev txhawb nqa ntawm tes. Lawv txoj kev tshawb fawb dav dav tsom mus rau kev daws qhov kev pheej hmoo tshem tawm, kov yeej ntau yam teeb meem, thiab ua kom zoo dua siRNA tsub zuj zuj hauv cov hom phiaj.
Txoj Kev Escape Challenge:
Kev khiav tawm ntawm endosomes thiab lysosomes yog ib kauj ruam tseem ceeb rau siRNA kom siv nws cov kev kho mob hauv hlwb. Prisys Biotech paub txog qhov tseem ceeb ntawm kev txhim kho siRNA khiav tawm ntawm endosomes thiab lysosomes. Siv lawv cov kev txawj ntse, lawv tsim txoj hauv kev tshiab kom nce siRNA kev khiav tawm, ua kom nws muaj peev xwm ua kom muaj kev sib koom tes. Tsis tas li ntawd, Prisys Biotech tshawb nrhiav cov receptors tshwj xeeb lossis ligands los pab txhawb kev xa khoom thiab kev khiav tawm hauv lub cev kom zoo.
Kev Sib Tw Ua Haujlwm:
Kev ua haujlwm hauv vivo ntawm siRNA yuav tsum muaj kev ruaj ntseg, muaj kev cuam tshuam, thiab kev nyab xeeb. Prisys Biotech tau mob siab rau optimizing siRNA stability, bioavailability, thiab ua tau zoo los ntawm chemically modifying siRNA molecules. Lawv leverage cov tshuaj hloov kho tshiab, xws li txhim kho kev ruaj ntseg los ntawm ESC + tshuaj hloov kho thiab qhia RNAi- ua rau cov qauv zoo li me me ncig cuam tshuam RNA (sciRNAs), asymmetric siRNA, thiab di-siRNA scaffolds. Cov txheej txheem tshiab no txhim kho siRNA kev ua tau zoo thiab pab kov yeej cov teeb meem cuam tshuam nrog kev tawm - cov phiaj xwm cuam tshuam thiab toxicity.
Prisys Biotech txoj kev koom tes:
Raws li CRO tshwj xeeb hauv kev tshawb fawb NHP, Prisys Biotech plays lub luag haujlwm tseem ceeb hauv kev nce qib siRNA kho. Lawv lub xeev- ntawm-- cov chaw tshawb fawb kos duab, nrog rau lawv cov kev txawj ntse hauv NHP cov tshuaj thiab kev soj ntsuam kev nyab xeeb, tso cai rau lawv muab kev nkag siab zoo rau kev txhim kho tshuaj siRNA. Prisys Biotech nquag koom tes nrog cov neeg koom tes hauv kev kawm thiab kev lag luam tshuaj, muab kev sib koom ua ke- txoj hauv kev txhim kho thiab txhawb kev txhais lus ntawm siRNA rau hauv kev siv tshuaj kho mob.
siRNA kev kho mob muaj peev xwm loj heev hauv kev hloov kho cov kab mob, tab sis lawv kuj nthuav tawm cov teeb meem tshwj xeeb. Prisys Biotech qhov kev txawj ntse hauv NHP kev tshawb fawb thiab lawv cov kev koom tes ua CRO tau tso lawv ua tus tseem ceeb hauv kev kov yeej cov teeb meem no. Los ntawm kev hais txog kev nkag mus, Kev khiav tawm, thiab kev ua tau zoo, Prisys Biotech ua lub luag haujlwm tseem ceeb hauv kev txhim kho siRNA thiab coj peb los ze zog rau yav tom ntej uas siRNA-raws li kev kho mob ua rau muaj kev cuam tshuam loj rau kev saib xyuas neeg mob.
